Antimicrobial Protection of the Mouse Testis: Synthesis of Defensins of the Cryptdin Family1

作者：Valérie Grandjean, Stéphane D. Vincent, Luc Martin, Minoo Rassoulzadegan, Franςois Cuzin · 发表于：Biology of Reproduction · 年份：1997 · DOI：10.1095/biolreprod57.5.1115 · 被引用次数：56 · 研究领域：Antimicrobial Peptides and Activities、Biochemical and Structural Characterization、RNA Interference and Gene Delivery

We report the synthesis by mouse testicular cells of antibiotic peptides related to the defensins secreted by the Paneth cells of the intestinal epithelium. A Sertoli cell-derived line (15P-1), Sertoli cells in primary cultures, and explanted testicular tissue in culture medium were observed to release protease-sensitive material with a broad-spectrum antibacterial activity. The activity of 15P-1 culture medium was increased 10- to 50-fold in the presence of fractions enriched in round spermatids and of nerve growth factor. Two series of results suggest that this activity may correspond to the release by testicular cells of defensin peptides, and specifically, of peptides of the cryptdin family first identified in the Paneth cells of intestinal crypts. First, a characteristic nucleotide sequence corresponding to the conserved first exon of the mouse cryptdin and cryptdin-related (CRS) genes was evidenced in the RNA of 15P-1 cells and of the testis. Second, immunohistochemical analysis demonstrated the presence of cryptdins of the cryp-1, -2, -3, -6 group in 15P-1 cells, and identified two distinct localizations in the testis. Inside the seminiferous tubule, these cryptdins were found accumulated in Sertoli cells at stages corresponding to the maturation of spermatids. In the interstitial space, Leydig cells also contained immunoreactive cryptdins.