Subtle genetic changes enhance virulence of methicillin resistant and sensitive Staphylococcus aureus

作者：Sarah K. Highlander, Kristina G. Hultén, Xiang Qin, Huaiyang Jiang, Shailaja Yerrapragada, Edward O. Mason, Yue Shang, Tiffany M. Williams, Régine M. Fortunov, Yamei Liu, Okezie Igboeli, Joseph F. Petrosino, Madhan R. Tirumalai, Akif Uzman, George E. Fox, Ana María Cárdenas, Donna M. Muzny, Lisa Hemphill, Yan Ding, Shannon Dugan, Peter R Blyth, Christian Buhay, Huyen Dinh, Alicia Hawes, Michael Holder, Christie Kovar, Sandra L. Lee, Wen Liu, Lynne V. Nazareth, Qiaoyan Wang, Jianling Zhou, Sheldon L. Kaplan, George M. Weinstock · 发表于：BMC Microbiology · 年份：2007 · DOI：10.1186/1471-2180-7-99 · 被引用次数：265 · 研究领域：Antimicrobial Resistance in Staphylococcus、Bacterial biofilms and quorum sensing、Streptococcal Infections and Treatments

BACKGROUND: Community acquired (CA) methicillin-resistant Staphylococcus aureus (MRSA) increasingly causes disease worldwide. USA300 has emerged as the predominant clone causing superficial and invasive infections in children and adults in the USA. Epidemiological studies suggest that USA300 is more virulent than other CA-MRSA. The genetic determinants that render virulence and dominance to USA300 remain unclear. RESULTS: We sequenced the genomes of two pediatric USA300 isolates: one CA-MRSA and one CA-methicillin susceptible (MSSA), isolated at Texas Children's Hospital in Houston. DNA sequencing was performed by Sanger dideoxy whole genome shotgun (WGS) and 454 Life Sciences pyrosequencing strategies. The sequence of the USA300 MRSA strain was rigorously annotated. In USA300-MRSA 2658 chromosomal open reading frames were predicted and 3.1 and 27 kilobase (kb) plasmids were identified. USA300-MSSA contained a 20 kb plasmid with some homology to the 27 kb plasmid found in USA300-MRSA. Two regions found in US300-MRSA were absent in USA300-MSSA. One of these carried the arginine deiminase operon that appears to have been acquired from S. epidermidis. The USA300 sequence was aligned with other sequenced S. aureus genomes and regions unique to USA300 MRSA were identified. CONCLUSION: USA300-MRSA is highly similar to other MRSA strains based on whole genome alignments and gene content, indicating that the differences in pathogenesis are due to subtle changes rather than to large-s...