Alterations in MurM, a Cell Wall Muropeptide Branching Enzyme, Increase High-Level Penicillin and Cephalosporin Resistance in Streptococcus pneumoniae

作者：Anthony Marius Smith, Keith P. Klugman · 发表于：Antimicrobial Agents and Chemotherapy · 年份：2001 · DOI：10.1128/aac.45.8.2393-2396.2001 · 被引用次数：91 · 研究领域：Pneumonia and Respiratory Infections、Antibiotic Resistance in Bacteria、Genomics and Phylogenetic Studies

We report that alteration in MurM, an enzyme involved in the biosynthesis of branched-stem cell wall muropeptides, is required for maximal expression of penicillin and cefotaxime resistance in the pneumococcus. Hungarian isolate 3191 (penicillin MIC, 16 microg/ml; cefotaxime MIC, 4 microg/ml) was a source of donor DNA in transformation experiments. Penicillin-binding protein DNA was insufficient to transform recipient strain R6 to full resistance. Further transformation with altered murM DNA was required for full expression of donor penicillin and cefotaxime resistance.