Empirical Analysis of Transcriptional Activity in the Arabidopsis Genome

作者：Kayoko Yamada, Jun Lim, Joseph M. Dale, Huaming Chen, Paul Shinn, Curtis J. Palm, Audrey M. Southwick, Hank C. Wu, Christopher M. Kim, Michelle Ly Thai Nguyen, Paul K. Pham, Rosa Cheuk, George Karlin-Newmann, Shirley X. Liu, Bao Lam, Hitomi Sakano, Troy Wu, Guixia Yu, Molly Miranda, Hong Quach, Matthew L. Tripp, Charlie H. Chang, Jeong Min Lee, Mitsue J. Toriumi, Marie M. H. Chan, Carolyn C. Tang, Courtney S. Onodera, Justine M. Deng, Kenji Akiyama, Yasser Ansari, Takahiro Arakawa, Jenny Banh, Fumika Banno, Leah Bowser, Shelise Y. Brooks, Piero Carninci, Qimin Chao, Nathan Choy, Akiko Enju, Andrew D. Goldsmith, Mani Gurjal, Nancy Fisher Hansen, Yoshihide Hayashizaki, Chanda Johnson-Hopson, Vickie Hsuan, Kei Iida, Meagan Karnes, Shehnaz Khan, Eric Koesema, Junko Ishida, Paul X. Jiang, Ted Jones, Jun Kawai, Asako Kamiya, Cristina C. Meyers, Maiko Nakajima, Mari Narusaka, Motoaki Seki, Tetsuya Sakurai, Masakazu Satou, Racquel Tamse, Maria Vaysberg, Erika K. Wallender, Cecilia Wong, Yuki Yamamura, Shiaulou Yuan, Kazuo Shinozaki, Ronald W. Davis, Athanasios Theologis, Joseph R. Ecker · 发表于：Science · 年份：2003 · DOI：10.1126/science.1088305 · 被引用次数：890 · 研究领域：Genomics and Phylogenetic Studies、Plant Molecular Biology Research、Photosynthetic Processes and Mechanisms

Functional analysis of a genome requires accurate gene structure information and a complete gene inventory. A dual experimental strategy was used to verify and correct the initial genome sequence annotation of the reference plant Arabidopsis. Sequencing full-length cDNAs and hybridizations using RNA populations from various tissues to a set of high-density oligonucleotide arrays spanning the entire genome allowed the accurate annotation of thousands of gene structures. We identified 5817 novel transcription units, including a substantial amount of antisense gene transcription, and 40 genes within the genetically defined centromeres. This approach resulted in completion of approximately 30% of the Arabidopsis ORFeome as a resource for global functional experimentation of the plant proteome.