Chromatographic Separation, and Characteristics of Nucleic Acids from HeLa Cells

作者：Lennart Philipson · 发表于：The Journal of General Physiology · 年份：1961 · DOI：10.1085/jgp.44.5.899 · 被引用次数：65 · 研究领域：Protein purification and stability、Carcinogens and Genotoxicity Assessment、Molecular Biology Techniques and Applications

The application of the phenol-duponol method to extraction of nucleic acids from HeLa cells is described. Chromatography of the phenol extract on an esterified bovine serum albumin column with a salt gradient of sodium chloride gives separation of soluble RNA, DNA, and two different high molecular RNA fractions. Ultracentrifugation of the DNA eluted from the column gives a sedimentation coefficient (s(20) (o),w) of 38, which agrees with ultracentrifugation data on the phenol extract. The eluted RNA appears polydisperse at low ionic strength, but at high ionic strength and after alcohol precipitation two fractions with the sedimentation coefficients of 16 and 25 to 29, respectively, were obtained.