Cyclic diguanylate (c‐di‐GMP) regulates Vibrio cholerae biofilm formation

作者：Anna D. Tischler, Andrew Camilli · 发表于：Molecular Microbiology · 年份：2004 · DOI：10.1111/j.1365-2958.2004.04155.x · 被引用次数：506 · 研究领域：Vibrio bacteria research studies、Antibiotic Resistance in Bacteria、Bacterial biofilms and quorum sensing

While studying virulence gene regulation in Vibrio cholerae during infection of the host small intestine, we identified VieA as a two-component response regulator that contributes to activating expression of cholera toxin. Here we report that VieA represses transcription of Vibrio exopolysaccharide synthesis (vps) genes involved in biofilm formation by a mechanism independent of its phosphorelay and DNA-binding activities. VieA controls the intracellular concentration of the cyclic nucleotide second messenger cyclic diguanylate (c-di-GMP) using an EAL domain that functions as a c-di-GMP phosphodiesterase. Two-dimensional thin layer chromatography of nucleotide extracts confirmed that VieA reduces the concentration of c-di-GMP, opposing the action of c-di-GMP synthetase proteins. Expression of unrelated V. cholerae c-di-GMP synthetase or phosphodiesterae proteins also modulated c-di-GMP concentration and vps gene expression. We propose that c-di-GMP synthetase and phosphodiesterase domain-containing proteins contribute to regulating biofilm formation by controlling c-di-GMP concentration.