Analysis of multiply antimicrobial-resistant isolates of Streptococcus pneumoniae from the United States

作者：Linda K. McDougal, Richard R. Facklam, Michael W. Reeves, Susan B. Hunter, Jana M. Swenson, Bertha C. Hill, Fred C. Tenover · 发表于：Antimicrobial Agents and Chemotherapy · 年份：1992 · DOI：10.1128/aac.36.10.2176 · 被引用次数：176 · 研究领域：Pneumonia and Respiratory Infections、Antibiotics Pharmacokinetics and Efficacy、Antibiotic Resistance in Bacteria

Streptococcus pneumoniae isolates resistant to penicillin, chloramphenicol, tetracycline and sulfamethoxazole-trimethroprim are being recovered with increasing frequency in the United States. We analyzed the penicillin-binding proteins (PBPs), multilocus enzyme electrophoresis (MLEE) genotypes, and ribotypes of 22 multiresistant serotype 23F isolates of S. pneumoniae from the United States and 1 isolate each from Spain and South Africa. Also included were seven multiresistant isolates of other serotypes, three penicillin-resistant but chloramphenicol-susceptible serotype 23F isolates, and two penicillin-susceptible isolates (one penicillin-susceptible isolate was serotype 23F). Fifteen of the 22 multiresistant isolates from the United States and the isolates from Spain and South Africa had identical PBP patterns, MLEE profiles, and ribotypes. Six of the remaining seven multiresistant isolates were related by PBP pattern, but demonstrated slightly different MLEE and/or ribotype profiles, possibly because of acquisition of additional resistance markers (four of the six isolates were also resistant to erythromycin). The remaining multiresistant serotype 23F isolate had a unique PBP pattern and ribotype and was only distantly related to the other pneumococcal isolates by MLEE analysis. The PBP patterns, MLEE profiles, and ribotypes of the multiresistant serotype 23F isolates were easily distinguished from those of six multiresistant isolates of other serotypes; three other penici...