Matrix‐assisted laser desorption/ionization mass spectrometry of nucleic acids with wavelengths in the ultraviolet and infrared

作者：Eckhard Nordhoff, Arnd Ingendoh, Rainer Cramer, A. Overberg, Bernd Stahl, Michael Karas, Franz Hillenkamp, P. F. Crain, Brian T. Chait · 发表于：Rapid Communications in Mass Spectrometry · 年份：1992 · DOI：10.1002/rcm.1290061212 · 被引用次数：296 · 研究领域：Mass Spectrometry Techniques and Applications、Analytical Chemistry and Chromatography、Metabolomics and Mass Spectrometry Studies

A number of different matrices have been tested and compared for ultraviolet and infrared (UV and IR) matrix-assisted laser desorption/ionization (MALDI) of oligodeoxyribonucleotides and mixtures thereof, as well as ribonucleic acids (tRNA from yeast and rRNA from E. coli). A new technique for removing alkali cations from nucleic acid samples during sample preparation on the sample support is demonstrated. The amount of oligonucleotide sample consumed during a typical measurement in IR-MALDI-MS was determined.