Differential staining of cartilage and bone in whole mouse fetuses by alcian blue and alizarin red S

作者：Michael J. McLeod · 发表于：Teratology · 年份：1980 · DOI：10.1002/tera.1420220306 · 被引用次数：623 · 研究领域：Molecular Biology Techniques and Applications

The procedure described by Inouye ('76) for the staining of full-term mouse fetal skeletons has been adapted for use with mouse embryos and fetuses of days 14-18 of gestation. The main adaptations for younger specimens involve a longer time in acetone, in lieu of skinning, and omission of the aqueous KOH step. These adaptations require more time but result in consistently good staining of intact specimens.