Topographical Location of H—Y Antigen on Mouse Spermatozoa by Immunoelectronmicroscopy

作者：Gloria C. Koo, Christopher W. Stackpole, Edward A. Boyse, Ulrich Hämmerling, Michael P. Lardis · 发表于：Proceedings of the National Academy of Sciences · 年份：1973 · DOI：10.1073/pnas.70.5.1502 · 被引用次数：101 · 研究领域：Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities、Sperm and Testicular Function、Animal Genetics and Reproduction

H-Y (male) antigen was visually located on mouse sperm by electron microscopy, by use of the indirect hybrid antibody method and tobacco mosaic virus as the visual marker. Labeling was achieved by centrifugation of the sperm through a discontinuous gradient consisting of alternating layers of immune reagents and wash solutions. Treated sperm were examined topographically by preparation of platinum-carbon replicas. Antigen was located mainly on the acrosomal cap of the sperm head.