Quantitative Comparisons of 16S rRNA Gene Sequence Libraries from Environmental Samples

作者：David R. Singleton, Michelle Furlong, Stephen L. Rathbun, William B. Whitman · 发表于：Applied and Environmental Microbiology · 年份：2001 · DOI：10.1128/aem.67.9.4374-4376.2001 · 被引用次数：624 · 研究领域：Environmental DNA in Biodiversity Studies、Molecular Biology Techniques and Applications、Genomics and Phylogenetic Studies

To determine the significance of differences between clonal libraries of environmental rRNA gene sequences, differences between homologous coverage curves, CX(D), and heterologous coverage curves, CXY(D), were calculated by a Cramér-von Mises-type statistic and compared by a Monte Carlo test procedure. This method successfully distinguished rRNA gene sequence libraries from soil and bioreactors and correctly failed to find differences between libraries of the same composition.