Derivation of Lymphoma Variants With Reduced Sensitivity to Plant Lectins2

作者：Robert Hyman, M LaCorbiere, Suzan Stavarek, Garth L. Nicolson · 发表于：JNCI Journal of the National Cancer Institute · 年份：1974 · DOI：10.1093/jnci/52.3.963 · 被引用次数：56 · 研究领域：Toxin Mechanisms and Immunotoxins、Transgenic Plants and Applications、Galectins and Cancer Biology

Variant murine lymphoma cell lines no longer sensitive to the cytotoxic action of lectin antilectin and complement were selected. Lectins were affinity-purified Ricinus communis and wheat germ agglutinins. The variant cell lines expressed 0.5–0.7 of the parental amount of total lectin-binding sites and showed a 10- to 100-fold decrease in sensitivity to the direct cytotoxic action of the lectin. There were no significant changes in other cellsurface determinants.