Cerium Oxide Nanoparticles Protect Cardiac Progenitor Cells from Oxidative Stress

作者：Francesca Pagliari, Corrado Mandoli, Giancarlo Forte, Eugenio Magnani, Stefania Pagliari, Giorgia Nardone, Silvia Licoccia, Marilena Minieri, Paolo Di Nardo, Enrico Traversa · 发表于：ACS Nano · 年份：2012 · DOI：10.1021/nn2048069 · 被引用次数：355 · 研究领域：Advanced Nanomaterials in Catalysis、Graphene and Nanomaterials Applications、Nanoparticles: synthesis and applications

Cardiac progenitor cells (CPCs) are a promising autologous source of cells for cardiac regenerative medicine. However, CPC culture in vitro requires the presence of microenvironmental conditions (a complex array of bioactive substance concentration, mechanostructural factors, and physicochemical factors) closely mimicking the natural cell surrounding in vivo, including the capability to uphold reactive oxygen species (ROS) within physiological levels in vitro. Cerium oxide nanoparticles (nanoceria) are redox-active and could represent a potent tool to control the oxidative stress in isolated CPCs. Here, we report that 24 h exposure to 5, 10, and 50 μg/mL of nanoceria did not affect cell growth and function in cardiac progenitor cells, while being able to protect CPCs from H(2)O(2)-induced cytotoxicity for at least 7 days, indicating that nanoceria in an effective antioxidant. Therefore, these findings confirm the great potential of nanoceria for controlling ROS-induced cell damage.