Adenovirus Expressing Human Interferon Inhibits Replication of Foot and Mouth Disease Virus and Reduces Fatal Rate in Mice

作者：Jia-Qi Chu, Su-Mi Kim, Kwang‐Nyeong Lee, Jae‐Ku Oem, Young-Joon Ko, Hyang-Sim Lee, Yong‐Joo Kim, Jee‐Yong Park, Jee‐Yong Park, Kwang-Jae Kim, Satya Parida, Yooni Oh, David J. Paton, Yi-Seok Joo, Byounghan Kim, Jong-Hyeon Park, Jong-Hyeon Park · 发表于：Journal of Bacteriology and Virology · 年份：2012 · DOI：10.4167/jbv.2012.42.3.224 · 被引用次数：2 · 研究领域：Animal Disease Management and Epidemiology、Viral Infections and Immunology Research、Vector-Borne Animal Diseases

Interferon is an important cytokine that plays a critical role in the initial host defense against viral infection. Recombinant human adenoviruses expressing human interferon- (Ad-HIFN) or pig interferon- fused with interleukin-18 (Ad-PIFN-IL18) were constructed and used to induce an early protective response against foot and mouth disease (FMD). To analyze the antiviral effect, bovine thyroid and porcine kidney IBRS-2 cells and ICR mice were treated with Ad-HIFN, Ad-PIFN-IL18, and cocktail of Ad-HIFN and Ad-PIFN-IL18. The survival rate of suckling mice was monitored after foot and mouth disease virus (FMDV) challenge following intra-peritoneal (IP) administration of appropriate adenovirus. Indirect antigen ELISA was performed to evaluate inhibition of FMDV replication following challenge with the FMDV O, A, or Asia 1 serotypes in vitro. These recombinant adenoviruses reduced the replication of FMDV in susceptible cells, thereby decreasing the fatality in mice, suggesting that they can be a useful control method for the early protection against FMD infection in livestock after field trial.