Maintaining perpetual synchrony in HeLa S3 culture

作者：William G. Thilly, Tanja Nowak, G. N. Wogan · 发表于：Biotechnology and Bioengineering · 年份：1974 · DOI：10.1002/bit.260160111 · 被引用次数：23 · 研究领域：Molecular Junctions and Nanostructures、Innovative Microfluidic and Catalytic Techniques Innovation、thermodynamics and calorimetric analyses

Abstract HeLa S3 cells in suspension culture are resynchronized in each successive generation by exposure to 0.25mM thymidine. The resynchronization approach appears to be superior to single‐ and double‐block techniques because the period of effective inhibition of DNA synthesis is significantly shortened. Gram quantities of highly synchronous viable cells have been produced on a daily basis.