Evidence that TolC is required for functioning of the Mar/AcrAB efflux pump of Escherichia coli

作者：Joe A. Fralick · 发表于：Journal of Bacteriology · 年份：1996 · DOI：10.1128/jb.178.19.5803-5805.1996 · 被引用次数：410 · 研究领域：Bacterial Genetics and Biotechnology、Antibiotic Resistance in Bacteria、Escherichia coli research studies

A study examining the influence of TolC on AcrA, AcrR, and MarR1 mutants indicates that functional TolC is required for the operation of the AcrAB efflux system and for the expression of the Mar phenotype. That the effect of TolC on the AcrAB pump is not regulatory in nature is shown by studies measuring the influence of a tolC::Tn10 insertion mutation on the expression of an acrA::lacZ reporter fusion. These results are compatible with the hypothesis that TolC is a component of the AcrAB efflux complex.