Voltage‐gated currents distinguish parvocellular from magnocellular neurones in the rat hypothalamic paraventricular nucleus

作者：Jason A. Luther, Jeffrey G. Tasker · 发表于：The Journal of Physiology · 年份：2000 · DOI：10.1111/j.1469-7793.2000.t01-1-00193.x · 被引用次数：184 · 研究领域：Neuroscience of respiration and sleep、Neuroendocrine regulation and behavior、Photoreceptor and optogenetics research

1. Magnocellular and parvocellular neurones of the hypothalamic paraventricular nucleus (PVN) differentially regulate pituitary hormone secretion and autonomic output. Previous experiments have suggested that magnocellular, or type I neurones, and parvocellular, or type II neurones, of the PVN express different electrophysiological properties. Whole-cell patch-clamp recordings were performed in hypothalamic slices to identify the voltage-gated currents responsible for the electrophysiological differences between type I and type II PVN neurones. 2. Type I neurones, which display transient outward rectification and lack a low-threshold spike (LTS), generated a large A-type K+ current (IA) (mean +/- s.e. m.: 1127.5 +/- 126.4 pA; range: 250-3600 pA; voltage steps to -25 mV) but expressed little or no T-type Ca2+ current (IT). Type II neurones, which lack transient outward rectification but often display an LTS, expressed a smaller IA (360.1 +/- 56.3 pA; range: 40-1100 pA; voltage steps to -25 mV), and 75 % of the type II neurones generated an IT (-402.5 +/- 166.9 pA; range: -90 to -2200 pA; at peak). 3. The voltage dependence of IA was shifted to more negative values in type I neurones compared to type II neurones. Thus, the activation threshold (-53.5 +/- 0.9 and -46.1 +/- 2.6 mV), the half-activation potential (-25 +/- 1.9 and -17.9 +/- 2.0 mV), the half-inactivation potential (-80.4 +/- 9.3 and -67.2 +/- 3.0 mV), and the potential at which the current became fully inactivated ...