The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit.

作者：Timothy J. Durfee, Kathleen Becherer, Phang‐Lang Chen, S.-H. Yeh, Yijing Yang, April E. Kilburn, W H Lee, Stephen J. Elledge · 发表于：Genes & Development · 年份：1993 · DOI：10.1101/gad.7.4.555 · 被引用次数：1460 · 研究领域：Cancer-related Molecular Pathways、Ubiquitin and proteasome pathways、Microtubule and mitosis dynamics

The retinoblastoma protein (p110RB) interacts with many cellular proteins in complexes potentially important for its growth-suppressing function. We have developed and used an improved version of the yeast two-hybrid system to isolate human cDNAs encoding proteins able to bind p110RB. One clone encodes a novel type 1 protein phosphatase catalytic subunit (PP-1 alpha 2), which differs from the originally defined PP-1 alpha by an amino-terminal 11-amino-acid insert. In vitro-binding assays demonstrated that PP-1 alpha isoforms preferentially bind the hypophosphorylated form of p110RB. Moreover, similar p110RB sequences are required for binding PP-1 alpha 2 and SV40 large T antigen. Cell cycle synchrony experiments revealed that this association occurs from mitosis to early G1. The implications of these findings on the regulation of both proteins are discussed.