Preparation of milligrams of pure enantiomers using analytical‐scale high‐performance liquid chromatography

作者：Gaoyuan Liu, David M. Goodall, Anna T. Hunter, Peter R. Massey · 发表于：Chirality · 年份：1994 · DOI：10.1002/chir.530060412 · 被引用次数：20 · 研究领域：Analytical Chemistry and Chromatography、Microfluidic and Capillary Electrophoresis Applications、Protein purification and stability

Abstract Pure enantiomers of an agrochemical process intermediate, (RS)‐1‐(4‐chlorophenyl)‐4,4‐dimethyl‐2‐(1 H ‐1,2,4‐triazol‐1‐yl)‐pentan‐3‐one ( 1 ), have been prepared on the milligram scale under overload chromatographic conditions on an analytical chiral column (250 × 4.6 mm i.d.). The effects of variation of temperature and mobile phase composition on retention factor, separation factor, and peak resolution have been investigated. Effects of flow rate, enantiomer ratio, sample concentration, and column load on productivity are also studied. Seven milligrams of the less retained (+)‐enantiomer and 5 mg of the (−)‐enantiomer were obtained from a single injection of 21 mg of (RS)‐ 1 . © 1994 Wiley‐Liss, Inc.