A Genome-Wide Transcriptional Analysis of the Mitotic Cell Cycle

作者：Raymond Jaihyun Cho, Michael Jay Campbell, Elizabeth A. Winzeler, Lars M. Steinmetz, Andrew R. Conway, Lisa M. Wodicka, Tyra G. Wolfsberg, Andrei E. Gabrielian, David Landsman, David J. Lockhart, Ronald W. Davis · 发表于：Molecular Cell · 年份：1998 · DOI：10.1016/s1097-2765(00)80114-8 · 被引用次数：2085 · 研究领域：Genomics and Chromatin Dynamics、Fungal and yeast genetics research、RNA Research and Splicing

Progression through the eukaryotic cell cycle is known to be both regulated and accompanied by periodic fluctuation in the expression levels of numerous genes. We report here the genome-wide characterization of mRNA transcript levels during the cell cycle of the budding yeast S. cerevisiae. Cell cycle-dependent periodicity was found for 416 of the 6220 monitored transcripts. More than 25% of the 416 genes were found directly adjacent to other genes in the genome that displayed induction in the same cell cycle phase, suggesting a mechanism for local chromosomal organization in global mRNA regulation. More than 60% of the characterized genes that displayed mRNA fluctuation have already been implicated in cell cycle period-specific biological roles. Because more than 20% of human proteins display significant homology to yeast proteins, these results also link a range of human genes to cell cycle period-specific biological functions.