Relationship between Load of Virus in Alveolar Macrophages from Human Immunodeficiency Virus Type 1-Infected Persons, Production of Cytokines, and Clinical Status

作者：Juan G. Sierra Madero, Zahra Toossi, David L. Hom, Candace K. Finegan, Eugene M. Hoenig, Elizabeth A. Rich · 发表于：The Journal of Infectious Diseases · 年份：1994 · DOI：10.1093/infdis/169.1.18 · 被引用次数：96 · 研究领域：HIV Research and Treatment、HIV-related health complications and treatments、Pneumocystis jirovecii pneumonia detection and treatment

The level of human immunodeficiency virus type 1 (HIV-1) in lymphocytes and mononuclear phagocytes (MP) from the blood and pulmonary alveoli from 14 HIV-1-infected subjects during early (asymptomatic) and late (AIDS) stages of disease and the relationship between virus burden in MP and cytokine expression were assessed. Among asymptomatic subjects, HIV-1 was undetectable or low in both blood monocytes and alveolar macrophages (AM). Among subjects with AIDS, there was a significant increase of HIV-1 in AM but not monocytes. The level of HIV-1 in blood lymphocytes was higher than in either monocytes or AM. AM (but not monocytes) expressed increased levels of lipopolysaccharide-stimulated cytokine mRNA (tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6) during both early and late stages of HIV-1 infection regardless of virus load. AM thus may serve as a reservoir for virus in late stages of disease yet contribute to the immunopathogenesis of lung disease in both early and late stages through increased cytokine expression.