Effects of Cinnamic Acid on Expression of Tissue Factor Induced by TNFα in Endothelial Cells and its Mechanisms

作者：Xiaofei Li, Zhibin Wen, Xiaofan He, Shilin He · 发表于：Journal of the Chinese Medical Association · 年份：2006 · DOI：10.1016/s1726-4901(09)70220-5 · 被引用次数：11 · 研究领域：Natural Antidiabetic Agents Studies、Wound Healing and Treatments、Tannin, Tannase and Anticancer Activities

BACKGROUND: To observe the effects of cinnamic acid (CINN) on the expression of tissue factor (TF) induced by tumor necrosis factor alpha (TNFalpha) in human umbilical vein endothelium-derived cell line (ECV304) and the related mechanisms. METHODS: Cellular TF activity was determined with 1-stage clotting assay in the presence or absence of anti-TF antibody or DEGR-Vlla. TF mRNA was examined by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). I-kappaB was measured by Western blot analysis. Immunohistochemical analysis was performed to evaluate the activation of NF-kappaB. RESULTS: CINN (500 microg/mL) significantly decreased the TF activity in ECV304 induced by TNFalpha (1000 U/mL) (p < 0.01) in a concentration-dependent fashion (r = -0.953, p < 0.05). CINN also inhibited the expression of TF mRNA induced by TNFalpha. Immunohistochemical analysis demonstrated NF-kappaB translocation from the cytoplasm to the nucleus, and Western blot analysis showed I-KB decrement in cytoplasm after treatment of endothelial cells with TNFalpha. CINN diminished these changes induced by TNFalpha. CONCLUSION: CINN is able to attenuate TNFa-induced TF expression of vascular endothelial cells by inhibiting NF-kappaB activation.