T1 α Protein is Developmentally Regulated and Expressed by Alveolar Type I Cells, Choroid Plexus, and Ciliary Epithelia of Adult Rats

作者：Mary C. Williams, Yuxia Cao, Anne Hinds, Arun K. Rishi, Antoinette Wetterwald · 发表于：American Journal of Respiratory Cell and Molecular Biology · 年份：1996 · DOI：10.1165/ajrcmb.14.6.8652186 · 被引用次数：145 · 研究领域：Neonatal Respiratory Health Research、Congenital Diaphragmatic Hernia Studies、Pediatric Hepatobiliary Diseases and Treatments

T1 alpha is the first marker gene known to be expressed in the adult lung solely by the alveolar type I epithelial cell. Previous studies showed that T1 alpha transcripts are abundant in early rat embryos where they are found in the nervous system and in the foregut and certain of its derivatives including the primitive lung. By mid- to late gestation T1 alpha messenger RNA (mRNA) expression is lost from neural tissues but appears to increase in the lung throughout fetal life. To determine whether the T1 alpha transcripts are translated into protein, especially in early embryos which sometimes express transcripts that are translationally silent, we performed immunohistochemistry on embryos and fetal tissues and analyzed certain tissues by western blotting using a monoclonal antibody against T1 alpha protein. T1 alpha protein is present at all sites that have previously been shown to express the mRNA and at similar developmental stages. As estimated from western blots, T1 alpha protein abundance peaks at about fetal day 16 in the brain and decreases thereafter to a relative level in the adult that is lower than that of the neural tube of the day 13 embryo. Relative protein abundance in the lung is very low, although detectable, on embryonic day 13 but increases slowly until fetal day 20 when there is a dramatic increase. At the time of birth, restriction to the type I cell is not complete and therefore must occur during postnatal lung development. Immunostaining reveals additi...