H19non coding RNA-derived miR-675 enhances tumorigenesis and metastasis of breast cancer cells by downregulating c-Cbl and Cbl-b

作者：Constance Vennin, Nathalie Spruyt, Fatima Zohra Dahmani, Sylvain Julien, François Bertucci, Pascal Finetti, Thierry Chassat, Roland P. Bourette, Xuefen Le Bourhis, Éric Adriaenssens · 发表于：Oncotarget · 年份：2015 · DOI：10.18632/oncotarget.4976 · 被引用次数：202 · 研究领域：MicroRNA in disease regulation、Cancer-related molecular mechanisms research、RNA modifications and cancer

// Constance Vennin 1,2 , Nathalie Spruyt 3 , Fatima Dahmani 3 , Sylvain Julien 1,2 , François Bertucci 4 , Pascal Finetti 4 , Thierry Chassat 5 , Roland P. Bourette 3 , Xuefen Le Bourhis 1,2 and Eric Adriaenssens 1,2 1 INSERM U908, Cell Plasticity and Cancer, F-59655, Villeneuve d’Ascq, France 2 University of Lille, F-59655, Villeneuve d’Ascq, France 3 CNRS UMR 8161, F-59021, Lille, France 4 Paoli-Calmettes Institute, Aix -Marseille University, F-13009, Marseille, France 5 PLETHA, Institut Pasteur Lille, F-59019, Lille, France Correspondence to: Eric Adriaenssens, email: // Keywords : H19, miRNA, breast cancer, CBL, tyrosine kinase receptor Received : April 28, 2015 Accepted : July 16, 2015 Published : July 22, 2015 Abstract H19 is a long non-coding RNA precursor of miR-675microRNA. H19 is increasingly described to play key roles in the progression and metastasis of cancers from different tissue origins. We have previously shown that the H19 gene is activated by growth factors and increases breast cancer cell invasion. In this study, we established H19 /miR-675 ectopic expression models of MDA-MB-231 breast cancer cells to further investigate the underlying mechanisms of H19 oncogenic action. We showed that overexpression of H19 /miR-675 enhanced the aggressive phenotype of breast cancer cells including increased cell proliferation and migration in vitro , and increased tumor growth and metastasis in vivo . Moreover, we identified ubiquitin ligase E3 family (c-Cbl and Cbl-b)...