Membrane Chromatography: Preparation and Applications to Protein Separation

作者：X. Zeng, Eli Ruckenstein · 发表于：Biotechnology Progress · 年份：1999 · DOI：10.1021/bp990120e · 被引用次数：209 · 研究领域：Protein purification and stability

As a result of the convective flow of solutes through porous membranes, membrane chromatography has a higher capture efficiency and a higher productivity than column chromatography and shows most promising industrial applications for the recovery, isolation, and purification of proteins and enzymes. This paper presents a comprehensive review of the methods for preparation of adsorptive membranes (such as surface modification, in situ copolymerization, direct formation from hydrophilic materials, and functionalized particulate-entrapped membranes) and deals particularly with novel macroporous chitin and chitosan membranes for protein separations developed by the authors.