Gene Targeting in Aspergillus fumigatus by Homologous Recombination Is Facilitated in a Nonhomologous End- Joining-Deficient Genetic Background

作者：Sven Krappmann, Christoph Sasse, Gerhard H. Braus · 发表于：Eukaryotic Cell · 年份：2006 · DOI：10.1128/ec.5.1.212-215.2006 · 被引用次数：306 · 研究领域：Fungal and yeast genetics research、Mycotoxins in Agriculture and Food、Antifungal resistance and susceptibility

The akuA gene encoding the Ku70 component of the nonhomologous end-joining machinery was deleted in the opportunistic pathogen Aspergillus fumigatus. No obvious phenotype could be assessed for the corresponding mutant strain but relative frequencies of homologous recombination were increased as deduced from targeting the laccase-encoding abr2 gene.