In Situ Probing of Newly Synthesized Peptidoglycan in Live Bacteria with Fluorescent D ‐Amino Acids

作者：Erkin Kuru, Velocity Hughes, Pamela Brown, Edward A. Hall, Srinivas Tekkam, Felipe Cava, Miguel Angel de Pedro, Yves V. Brun, Michael S. VanNieuwenhze · 发表于：Angewandte Chemie International Edition · 年份：2012 · DOI：10.1002/anie.201206749 · 被引用次数：680 · 研究领域：Bacteriophages and microbial interactions、Bacterial Genetics and Biotechnology、RNA and protein synthesis mechanisms

Tracking a bug's life: Peptidoglycan (PG) of diverse bacteria is labeled by exploiting the tolerance of cells for incorporating different non-natural D-amino acids. These nontoxic D-amino acids preferably label the sites of active PG synthesis, thereby enabling fine spatiotemporal tracking of cell-wall dynamics in phylogenetically and morphologically diverse bacteria. HCC = 7-hydroxycoumarin, NBD = 7-nitrobenzofurazan, TAMRA = carboxytetramethylrhodamine.