Transcriptional Maps of 10 Human Chromosomes at 5-Nucleotide Resolution

作者：Jill Cheng, Philipp Kapranov, Jörg Drenkow, Sujit Dike, Shane Brubaker, Sandeep A. Patel, Jeffrey C. Long, David L. Stern, Hari Tammana, Gregg A. Helt, Victor I. Sementchenko, Antonio Piccolboni, Stefan Bekiranov, Dione K. Bailey, Madhavan Ganesh, Srinka Ghosh, Ian Bell, Daniela Se Gerhard, T Gingeras · 发表于：Science · 年份：2005 · DOI：10.1126/science.1108625 · 被引用次数：1231 · 研究领域：Chromosomal and Genetic Variations、Genomic variations and chromosomal abnormalities、Genomics and Chromatin Dynamics

Sites of transcription of polyadenylated and nonpolyadenylated RNAs for 10 human chromosomes were mapped at 5-base pair resolution in eight cell lines. Unannotated, nonpolyadenylated transcripts comprise the major proportion of the transcriptional output of the human genome. Of all transcribed sequences, 19.4, 43.7, and 36.9% were observed to be polyadenylated, nonpolyadenylated, and bimorphic, respectively. Half of all transcribed sequences are found only in the nucleus and for the most part are unannotated. Overall, the transcribed portions of the human genome are predominantly composed of interlaced networks of both poly A+ and poly A- annotated transcripts and unannotated transcripts of unknown function. This organization has important implications for interpreting genotype-phenotype associations, regulation of gene expression, and the definition of a gene.