Estimating telomere length from whole genome sequence data

作者：Zhihao Ding, Massimo Mangino, Abraham Aviv, Tim D. Spector, Richard M. Durbin · 发表于：Nucleic Acids Research · 年份：2014 · DOI：10.1093/nar/gku181 · 被引用次数：251 · 研究领域：Telomeres, Telomerase, and Senescence、Epigenetics and DNA Methylation、Mitochondrial Function and Pathology

Telomeres play a key role in replicative ageing and undergo age-dependent attrition in vivo. Here, we report a novel method, TelSeq, to measure average telomere length from whole genome or exome shotgun sequence data. In 260 leukocyte samples, we show that TelSeq results correlate with Southern blot measurements of the mean length of terminal restriction fragments (mTRFs) and display age-dependent attrition comparably well as mTRFs.