The Transcriptional Program of Sporulation in Budding Yeast

作者：Shelley Chu, Joseph L. DeRisi, Michael B. Eisen, Jon Mulholland, David Botstein, Patrick O. Brown, Ira Herskowitz · 发表于：Science · 年份：1998 · DOI：10.1126/science.282.5389.699 · 被引用次数：1888 · 研究领域：Fungal and yeast genetics research、Genomics and Chromatin Dynamics、Plant tissue culture and regeneration

Diploid cells of budding yeast produce haploid cells through the developmental program of sporulation, which consists of meiosis and spore morphogenesis. DNA microarrays containing nearly every yeast gene were used to assay changes in gene expression during sporulation. At least seven distinct temporal patterns of induction were observed. The transcription factor Ndt80 appeared to be important for induction of a large group of genes at the end of meiotic prophase. Consensus sequences known or proposed to be responsible for temporal regulation could be identified solely from analysis of sequences of coordinately expressed genes. The temporal expression pattern provided clues to potential functions of hundreds of previously uncharacterized genes, some of which have vertebrate homologs that may function during gametogenesis.