Ginsenoside Rg1 protects rat cardiomyocyte from hypoxia/reoxygenation oxidative injury via antioxidant and intracellular calcium homeostasis

作者：Dan Zhu, Lei Wu, Chunrong Li, Xiaowei Wang, Yong‐Jie Ma, Zhi‐yin Zhong, Hai‐Bao Zhao, Jian Cui, Si‐Fan Xun, Xiulan Huang, Zhe Zhou, Shengqi Wang · 发表于：Journal of Cellular Biochemistry · 年份：2009 · DOI：10.1002/jcb.22233 · 被引用次数：133 · 研究领域：Ginseng Biological Effects and Applications、Electron Spin Resonance Studies、Traditional Chinese Medicine Analysis

Ginsenoside Rg1 is a major active ingredient of Panax notoginseng radix which has demonstrated a number of pharmacological actions including a cardioprotective effect in vivo. This study investigated the protective effect and mechanism of ginsenoside Rg1 in cardiomyocytes hypoxia/reoxygenation (H/R) model. Pretreatment with ginsenoside Rg1 (60-120 microM) reduced lactate dehydrogenase release and increased cell viability in a dose-dependent manner. Fluorescence analysis demonstrated ginsenoside Rg1 reduced intracellular ROS and suppressed the intracellular [Ca(2+)] level. Cell lysate detected an increase of T-SOD, CAT, and GSH levels. The myocardial protection of ginsenoside Rg1 during H/R is partially due to its antioxidative effect and intracellular calcium homeostasis.