Isolation and characterization of abscisic acid‐deficient Arabidopsis mutants at two new loci

作者：Karen M. Léon‐Kloosterziel, Marta Álvarez Gil, Gerda J. Ruijs, Steven E. Jacobsen, Neil E. Olszewski, Steven H. Schwartz, Jan A. D. Zeevaart, Maarten Koornneef · 发表于：The Plant Journal · 年份：1996 · DOI：10.1046/j.1365-313x.1996.10040655.x · 被引用次数：481 · 研究领域：Plant Molecular Biology Research、Plant Stress Responses and Tolerance、Plant nutrient uptake and metabolism

Novel Arabidopsis mutants with lowered levels of endogenous abscisic acid (ABA) were isolated. These were selected in a screen for germination in the presence of the gibberellin biosynthesis inhibitor paclobutrazol. Another mutant was isolated in a screen for NaCl tolerance. The ABA-deficiency was caused by two monogenic, recessive mutations, aba2 and aba3, that were both located on chromosome 1. The mutants showed a phenotype that is known to be characteristic for ABA-deficiency: a reduced seed dormancy and excessive water loss, leading to a wilty phenotype. Double mutant analysis, combining different aba mutations, indicated the leaky nature of the mutations.