Role of Fibronectin in the Prevention of Adherence of Pseudomonas aeruginosa to Buccal Cells

作者：Donald E. Woods, David C. Straus, WALDEMAR G. JOHANSON, J A Bass · 发表于：The Journal of Infectious Diseases · 年份：1981 · DOI：10.1093/infdis/143.6.784 · 被引用次数：229 · 研究领域：Cystic Fibrosis Research Advances、Bacterial Infections and Vaccines、Inhalation and Respiratory Drug Delivery

Recent evidence suggests that colonization of the upper respiratory tract by gram-negative bacilli is mediated by adherence to regional epithelial cells. Buccal epithelial cells were obtained for study from 12 seriously ill patients, all of whom were colonized with Pseudomonas aeruginosa. In comparison to cells from uncolonized controls, cells obtained from these patients attached significantly more P. aeruginosa organisms during incubation in vitro. Although the sialic acid content of colonized patients' cells was less than that of controls' cells, removal of sialic acid from normal cells with neuraminidase did not increase bacillary adherence. Trypsinization of normal cells increased bacillary adherence and significantly reduced the amount of fibronectin on the cell surface. Both trypsinized normal cells and cells recovered from seriously ill colonized patients attached large numbers of P. aeruginosa organisms in vitro and demonstrated decreased fibronectin on the cell surface by immunofluorescent staining. These findings suggest that the host alteration associated with increased susceptibility to adherence by P. aeruginosa is the loss of fibronectin from the cell surface.