In vitro construction of bacteriophage lambda carrying segments of the Escherichia coli chromosome: selection of hybrids containing the gene for DNA ligase.

作者：JOHN R. CAMERON, Sharon M. Panasenko, I Lehman, Ronald W. Davis · 发表于：Proceedings of the National Academy of Sciences · 年份：1975 · DOI：10.1073/pnas.72.9.3416 · 被引用次数：141 · 研究领域：Bacteriophages and microbial interactions、Bacterial Genetics and Biotechnology、Microbial Metabolic Engineering and Bioproduction

DNA from lambdagt-lambdaB bacteriophage was cleaved with EcoRI endonuclease and fragments from EcoRI-digested E. coli DNA were inserted. This DNA was used to infect E. coli, and phages containing the gene for DNA ligase were isolated by genetic selection. Two different hybrids were found with the same E. coli segment inserted in opposite orientations. Both hybrids produced similar levels of ligase as measured in crude extracts of infected cells.