Sendai Virus Blocks Alpha Interferon Signaling to Signal Transducers and Activators of Transcription

作者：Takayuki Komatsu, Kenji Takeuchi, Junko Yokoo, Yukië Tanaka, Bin Gotoh · 发表于：Journal of Virology · 年份：2000 · DOI：10.1128/jvi.74.5.2477-2480.2000 · 被引用次数：109 · 研究领域：Viral Infections and Vectors、interferon and immune responses、Viral gastroenteritis research and epidemiology

We demonstrate here that Sendai virus (SeV) blocks alpha interferon (IFN-alpha) signaling to signal transducers and activators of transcription (STATs) in HeLa cells. IFN-alpha-stimulated tyrosine phosphorylation of STATs and subsequent formation of the IFN-stimulated gene factor 3 transcription complex were inhibited in SeV-infected cells, resulting in inefficient induction of IFN-stimulated gene products. None of the components of the signaling pathway-type I IFN receptor subunits Jak1, Tyk2, Stat1, Stat2, and p48-was degraded. Moreover, tyrosine phosphorylation of Jak1 in response to IFN-alpha was unaffected at the early phase of infection, suggesting that oligomerization of the receptor subunits proceeded normally. In contrast to Jak1, IFN-alpha-stimulated tyrosine phosphorylation of Tyk2 was partially inhibited. Therefore, this partial inhibition of activation of Tyk2 probably contributes to the subsequent failure in the activation of STATs.