Selective Depletion of Rat Neutrophils by In Vivo Administration of a Monoclonal Antibody

作者：Sakae Sekiya, Seiji Gotoh, Takao Yamashita, Tadashi Watanabe, Susumu Saitoh, Fujiro Sendo · 发表于：Journal of Leukocyte Biology · 年份：1989 · DOI：10.1002/jlb.46.2.96 · 被引用次数：83 · 研究领域：Immune Response and Inflammation、Inflammatory mediators and NSAID effects、Cell Adhesion Molecules Research

A monoclonal antibody (RP-3) that depletes rat neutrophils selectively in vivo was developed by hybridization of mouse myeloma cells (P3-X63.Ag8.653) and spleen cells of BALB/c mice sensitized with peritoneal neutrophils of WKA/Hok rats. RP-3 reacted with rat neutrophils but not with lymphocytes, macrophages, natural killer cells, basophils, eosinophils, or tissues of various organs. The mitogenic responsiveness to concanavalin A (ConA), phytohemagglutinin (PHA), and lipopolysaccharide (LPS) of rats given RP-3 was not significantly different from that of normal rats. Administration of RP-3 into the peritoneal cavity of rats that had been kept under specific pathogen-free (SPF) or clean conditions induced selective depletion of circulating neutrophils to under 100/mm3 (0.5% WBC). The numbers of monocytes, lymphocytes, and platelets were not changed. Administration of 2 ml of RP-3 reduced blood neutrophils to under 100/mm3 for approximately 24 h, and administration of 1 ml caused depletion for approximately 12 h.