Specific Targeting, Cell Sorting, and Bioimaging with Smart Magnetic Silica Core–Shell Nanomaterials

作者：Tae‐Jong Yoon, Kyeong Nam Yu, Eunha Kim, Jun Sung Kim, Byung Geol Kim, Sang-Hyun Yun, Byeong‐Hyeok Sohn, Myung‐Haing Cho, Jin‐Kyu Lee, Seung Bum Park · 发表于：Small · 年份：2005 · DOI：10.1002/smll.200500360 · 被引用次数：307 · 研究领域：Advanced biosensing and bioanalysis techniques、Nanoparticle-Based Drug Delivery、Advanced Biosensing Techniques and Applications

Organic dye-incorporated smart silica-coated core–shell magnetic nanoparticles (MNP@SiO2) having dual-functionality (-PEG/NH2, see Figure) were easily fabricated, and the amine moieties on the NP surface were modified with maleimide functionality for specific covalent immobilization of biopolymers and bioactive small molecules. The sequence-independent immobilization of antibodies (Ab) is highlighted; Ab-modified MNP@SiO2 particles exhibit specific recognition for floating tumor cells or target the membrane of adherent breast cancer cells.