Production, purification and characterization of hemolysins from Listeria ivanovii and Listeria monocytogenes Sv4b

作者：Jürgen Kreft, Dorothee Funke, Albert Haas, Friedrich Lottspeich, Werner Goebel · 发表于：FEMS Microbiology Letters · 年份：1989 · DOI：10.1111/j.1574-6968.1989.tb03298.x · 被引用次数：47 · 研究领域：Listeria monocytogenes in Food Safety、Microbial Inactivation Methods、Essential Oils and Antimicrobial Activity

In culture supernatants of both Listeria ivanovii and Listeria monocytogenes Sv4b, for the first time a hemolysin of molecular weight 58 kDa was identified, which had all the characteristics of an SH-activated cytolysin, and which was therefore identified as listeriolysin O (LLO). In the case of L. ivanovii a second major supernatant protein of molecular weight 24 kDa co-purified with LLO. However, the function of this protein has to be determined. In culture supernatants of L. ivanovii a sphingomyelinase and a lecithinase activity could be detected, both enzymatic activities together contributing to the pronounced hemolysis caused by L. ivanovii. The N-terminal amino acid sequences of LLO and the 24 kDa from L. ivanovii are shown.