Growth, Adipose, Brain, and Skin Alterations Resulting from Targeted Disruption of the Mouse Peroxisome Proliferator-Activated Receptor β(δ)

作者：Jeffrey M. Peters, Susanna S.T. Lee, Wen Li, Jerrold Michael Ward, Oksana Gavrilova, Carrie A. Everett, Marc L. Reitman, Lynn D. Hudson, Frank J. Gonzalez · 发表于：Molecular and Cellular Biology · 年份：2000 · DOI：10.1128/mcb.20.14.5119-5128.2000 · 被引用次数：632 · 研究领域：Peroxisome Proliferator-Activated Receptors、Adipose Tissue and Metabolism、Adipokines, Inflammation, and Metabolic Diseases

To determine the physiological roles of peroxisome proliferator-activated receptor beta (PPARbeta), null mice were constructed by targeted disruption of the ligand binding domain of the murine PPARbeta gene. Homozygous PPARbeta-null term fetuses were smaller than controls, and this phenotype persisted postnatally. Gonadal adipose stores were smaller, and constitutive mRNA levels of CD36 were higher, in PPARbeta-null mice than in controls. In the brain, myelination of the corpus callosum was altered in PPARbeta-null mice. PPARbeta was not required for induction of mRNAs involved in epidermal differentiation induced by O-tetradecanoylphorbol-13-acetate (TPA). The hyperplastic response observed in the epidermis after TPA application was significantly greater in the PPARbeta-null mice than in controls. Inflammation induced by TPA in the skin was lower in wild-type mice fed sulindac than in similarly treated PPARbeta-null mice. These results are the first to provide in vivo evidence of significant roles for PPARbeta in development, myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation.