Quantitative Trace Analysis of Complex Mixtures Using SABRE Hyperpolarization

作者：Nan Eshuis, Bram J. A. van Weerdenburg, Martin C. Feiters, Floris P. J. T. Rutjes, Sybren S. Wijmenga, Marco Tessari · 发表于：Angewandte Chemie International Edition · 年份：2014 · DOI：10.1002/anie.201409795 · 被引用次数：110 · 研究领域：Advanced NMR Techniques and Applications、Electron Spin Resonance Studies、Solid-state spectroscopy and crystallography

Signal amplification by reversible exchange (SABRE) is an emerging nuclear spin hyperpolarization technique that strongly enhances NMR signals of small molecules in solution. However, such signal enhancements have never been exploited for concentration determination, as the efficiency of SABRE can strongly vary between different substrates or even between nuclear spins in the same molecule. The first application of SABRE for the quantitative analysis of a complex mixture is now reported. Despite the inherent complexity of the system under investigation, which involves thousands of competing binding equilibria, analytes at concentrations in the low micromolar range could be quantified from single-scan SABRE spectra using a standard-addition approach.