DNA barcoding in Mucorales : an inventory of biodiversity

作者：Grit Walther, Julia Pawłowska, Ana Alastruey‐Izquierdo, Marta Wrzosek, J. L. Rodriguez-Tudela, Somayeh Dolatabadi, A. Chakrabarti, Sybren de Hoog · 发表于：Persoonia - Molecular Phylogeny and Evolution of Fungi · 年份：2013 · DOI：10.3767/003158513x665070 · 被引用次数：275 · 研究领域：Plant Pathogens and Fungal Diseases、Mycorrhizal Fungi and Plant Interactions、Fungal Biology and Applications

The order Mucorales comprises predominantly fast-growing saprotrophic fungi, some of which are used for the fermentation of foodstuffs but it also includes species known to cause infections in patients with severe immune or metabolic impairments. To inventory biodiversity in Mucorales ITS barcodes of 668 strains in 203 taxa were generated covering more than two thirds of the recognised species. Using the ITS sequences, Molecular Operational Taxonomic Units were defined by a similarity threshold of 99 %. An LSU sequence was generated for each unit as well. Analysis of the LSU sequences revealed that conventional phenotypic classifications of the Mucoraceae are highly artificial. The LSU- and ITS-based trees suggest that characters, such as rhizoids and sporangiola, traditionally used in mucoralean taxonomy are plesiomorphic traits. The ITS region turned out to be an appropriate barcoding marker in Mucorales. It could be sequenced directly in 82 % of the strains and its variability was sufficient to resolve most of the morphospecies. Molecular identification turned out to be problematic only for the species complexes of Mucor circinelloides, M. flavus, M. piriformis and Zygorhynchus moelleri. As many as 12 possibly undescribed species were detected. Intraspecific variability differed widely among mucorealean species ranging from 0 % in Backusella circina to 13.3 % in Cunninghamella echinulata. A high proportion of clinical strains was included for molecular identification. Clin...