Cascade Reactions in Multicompartmentalized Polymersomes

作者：Ruud J. R. W. Peters, Maïté Marguet, Sébastien Marais, Marco W. Fraaije, Jan C. M. van Hest, Sébastien Lecommandoux · 发表于：Angewandte Chemie International Edition · 年份：2013 · DOI：10.1002/anie.201308141 · 被引用次数：566 · 研究领域：Supramolecular Self-Assembly in Materials、Supramolecular Chemistry and Complexes、biodegradable polymer synthesis and properties

Enzyme-filled polystyrene-b-poly(3-(isocyano-L-alanyl-aminoethyl)thiophene) (PS-b-PIAT) nanoreactors are encapsulated together with free enzymes and substrates in a larger polybutadiene-b-poly(ethylene oxide) (PB-b-PEO) polymersome, forming a multicompartmentalized structure, which shows structural resemblance to the cell and its organelles. An original cofactor-dependent three-enzyme cascade reaction is performed, using either compatible or incompatible enzymes, which takes place across multiple compartments.