Major Proteome Variations Associated with Cherry Tomato Pericarp Development and Ripening

作者：Mireille Faurobert, Christina Mihr, Nadia Bertin, Tomasz Andrzej Pawłowski, Luc Négroni, Nicolas Sommerer, Mathilde Causse · 发表于：PLANT PHYSIOLOGY · 年份：2007 · DOI：10.1104/pp.106.092817 · 被引用次数：260 · 研究领域：Antioxidant Activity and Oxidative Stress、Advanced Proteomics Techniques and Applications、Plant Reproductive Biology

Tomato (Solanum lycopersicum) is a model plant for studying fleshy fruit development. Several genetic and molecular approaches have been developed to increase our knowledge about the physiological basis of fruit growth, but very few data are yet available at the proteomic level. The main stages of fruit development were first determined through the dynamics of fruit diameter and pericarp cell number. Then, total proteins were extracted from pericarp tissue at six relevant developmental stages and separated by two-dimensional gel electrophoresis. Protein patterns were markedly different between stages. Proteins showing major variations were monitored. We identified 90 of 1,791 well-resolved spots either by matrix-assisted laser-desorption ionization time-of-flight peptide mass fingerprinting or liquid chromatography-mass spectrometry sequencing and expressed sequence tag database searching. Clustered correlation analysis results pointed out groups of proteins with similar expression profiles during fruit development. In young fruit, spots linked to amino acid metabolism or protein synthesis were mainly expressed during the cell division stage and down-regulated later. Some spots linked to cell division processes could be identified. During the cell expansion phase, spots linked to photosynthesis and proteins linked to cell wall formation transiently increased. In contrast, the major part of the spots related to C compounds and carbohydrate metabolism or oxidative processes wer...