Recognition by Elam-1 of the Sialyl-Le x Determinant on Myeloid and Tumor Cells

作者：Gerd Walz, Alejandro A. Aruffo, Waldemar Kolanus, Michael P. Bevilacqua, Brian Seed · 发表于：Science · 年份：1990 · DOI：10.1126/science.1701275 · 被引用次数：968 · 研究领域：Glycosylation and Glycoproteins Research、Proteoglycans and glycosaminoglycans research、Cell Adhesion Molecules Research

Endothelial leukocyte adhesion molecule-1 (ELAM-1) is an endothelial cell adhesion molecule that allows myeloid cells to attach to the walls of blood vessels adjacent to sites of inflammation. ELAM-1 recognizes the sialyl-Lewis X (sialyl-Lex) determinant, NeuAc alpha 2-3Gal beta 1-4(Fuc alpha 1-3)GlcNAc-, a granulocyte carbohydrate also found on the surface of some tumor cell lines. Binding of myeloid cells to soluble ELAM-1 is inhibited by a monoclonal antibody recognizing sialyl-Lex or by proteins bearing sialyl-Lex, some of which may participate in humoral regulation of myeloid cell adhesion. Stimulated granulocytes also release an inhibitor of ELAM-1 binding that can be selectively adsorbed by monoclonal antibody to sialyl-Lex.