Subcellular compartmentalization of saccharide moieties in cultured normal and malignant cells.

作者：Ismo Virtanen, Peter Ekblom, P Laurila · 发表于：The Journal of Cell Biology · 年份：1980 · DOI：10.1083/jcb.85.2.429 · 被引用次数：258 · 研究领域：Glycosylation and Glycoproteins Research、Toxin Mechanisms and Immunotoxins、Erythrocyte Function and Pathophysiology

We studied subcellular localization of saccharide moieties in cultured normal and malignant cells fixed in paraformaldehyde and treated with a nonionic detergent, using lectins specific for various surgar residues as probes in fluorescence microscopy. In normal cells, concanavalin A and Lens culinaris agglutinin, specific for mannose-rich carbohydrate cores in glycoproteins, labeled the endoplasmic reticulum as a wide perinuclear region. Other lectins, on the other hand, stained the Golgi apparatus as a juxtanuclear reticular structure. A similar compartmentalization was also seen in all malignant cells studied, although the Golgi apparatus in these cells was distinctly vesicular in appearance. Our results indicate that saccharide moieties in both normal and malignant cells are similarly compartmentalized, and thus speak in favor of a unidirectional subcellular flow for both membrane and secreted glycoconjugates.