Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform

作者：Frédéric Mallard, Bor Luen Tang, Thierry Galli, Danièle Tenza, Agnès Saint‐Pol, Yue Xu, Claude Antony, Wanjin Hong, Bruno Goud, Ludger Johannes · 发表于：The Journal of Cell Biology · 年份：2002 · DOI：10.1083/jcb.200110081 · 被引用次数：543 · 研究领域：Cellular transport and secretion、Lysosomal Storage Disorders Research、Pancreatic function and diabetes

The molecular mechanisms underlying early/recycling endosomes-to-TGN transport are still not understood. We identified interactions between the TGN-localized putative t-SNAREs syntaxin 6, syntaxin 16, and Vti1a, and two early/recycling endosomal v-SNAREs, VAMP3/cellubrevin, and VAMP4. Using a novel permeabilized cell system, these proteins were functionally implicated in the post-Golgi retrograde transport step. The function of Rab6a' was also required, whereas its closely related isoform, Rab6a, has previously been implicated in Golgi-to-endoplasmic reticulum transport. Thus, our study shows that membrane exchange between the early endocytic and the biosynthetic/secretory pathways involves specific components of the Rab and SNARE machinery, and suggests that retrograde transport between early/recycling endosomes and the endoplasmic reticulum is critically dependent on the sequential action of two members of the Rab6 subfamily.