Analysis of BIOLOG GN Substrate Utilization Patterns by Microbial Communities

作者：Kornelia Smalla, Ute Wachtendorf, Holger Heuer, Wen‐Tso Liu, Larry J. Forney · 发表于：Applied and Environmental Microbiology · 年份：1998 · DOI：10.1128/aem.64.4.1220-1225.1998 · 被引用次数：403 · 研究领域：Microbial Community Ecology and Physiology、Wastewater Treatment and Nitrogen Removal、Microbial Fuel Cells and Bioremediation

BIOLOG GN plates are increasingly used to characterize microbial communities by determining the ability of the communities to oxidize various carbon sources. Studies were done to determine whether the BIOLOG GN plate assay accurately reflects the catabolic potential of the inoculum used. To gain insight into which populations of microbial communities contribute to the BIOLOG patterns, denaturing gradient gel electrophoresis and temperature gradient gel electrophoresis (TGGE) were used to assess the diversity of ribotypes in the inocula and individual wells of BIOLOG plates following incubation. These studies were done with microbial communities from the rhizosphere of potatoes and an activated sludge reactor fed with glucose and peptone. TGGE analyses of BIOLOG wells inoculated with cell suspensions from the potato rhizosphere revealed that, compared with the inoculum, there was a decrease in the number of 16S rRNA gene fragments obtained from various wells, as well as a concomitant loss of populations that had been numerically dominant in the inoculum. The dominant fragments in TGGE gels could be assigned to the gamma subclass of the class Proteobacteria, suggesting that fast-growing bacteria adapted to high substrate concentrations were numerically dominant in the wells and may have been primarily responsible for the patterns of substrate use that were observed. Similarly, the community structure changed in wells inoculated with cells from activated sludge; one or more popu...