Bone marrow contributes to renal parenchymal turnover and regeneration

作者：Richard Poulsom, Stuart John Forbes, Kairbaan Hodivala‐Dilke, Eoin G. Ryan, Susannah M. Wyles, Sobana Navaratnarasah, Rosemary E. Jeffery, Toby Hunt, Malcolm Ronald Alison, Terence H Cook, Charles Dickson Pusey, Nicholas A. Wright · 发表于：The Journal of Pathology · 年份：2001 · DOI：10.1002/path.976 · 被引用次数：648 · 研究领域：Renal and related cancers、Mesenchymal stem cell research、Pluripotent Stem Cells Research

In order to establish whether extra-renal cells contribute to the turnover and repair of renal tissues, this study examined kidneys of female mice that had received a male bone marrow transplant and kidney biopsies from male patients who had received kidney transplants from female donors. By using in situ hybridization to detect Y-chromosomes it could be demonstrated that circulating stem cells frequently engraft into the kidney and differentiate into renal parenchymal cells. In the human renal grafts it was confirmed that some of the recipient-derived cells within the kidney exhibited a tubular epithelial phenotype, by combining in situ hybridization with immunostaining for the epithelial markers CAM 5.2 and the lectin Ulex europaeus. Female mouse recipients of male bone marrow grafts showed co-localization of Y-chromosomes and tubular epithelial markers Ricinus communis and Lens culinaris, and a specific cytochrome P450 enzyme (CYP1A2) indicating an appropriate functional capability of clustered newly formed marrow-derived tubular epithelial cells. Y-chromosome-containing cells were observed within glomeruli, with morphology and location appropriate for podocytes. Within the murine kidney, these Y-chromosome-positive cells were negative for the mouse macrophage marker F4/80 antigen and leukocyte common antigen, but were vimentin-positive. The presence of bone marrow-derived cells was noted in both histologically normal mouse kidneys and in human transplanted kidneys sufferi...