Reduction of periodontal pathogens adhesion by antagonistic strains

作者：C.G. van Hoogmoed, Gésinda I. Geertsema-Doornbusch, Wim Teughels, Marc Quirynen, Henk J. Busscher, H.C. van der Mei · 发表于：Oral Microbiology and Immunology · 年份：2007 · DOI：10.1111/j.1399-302x.2007.00388.x · 被引用次数：80 · 研究领域：Oral microbiology and periodontitis research、Bacterial biofilms and quorum sensing、Endodontics and Root Canal Treatments

INTRODUCTION: Periodontitis results from a shift in the subgingival microflora into a more pathogenic direction with Porphyromonas gingivalis, Prevotella intermedia, and Actinobacillus actinomycetemcomitans considered as periodontopathogens. In many cases, treatment procures only a temporary shift towards a less pathogenic microflora. An alternative treatment could be the deliberate colonization of pockets with antagonistic microorganisms to control the adhesion of periodontopathogens. The aim of this study was to identify bacterial strains that reduce adhesion of periodontopathogens to surfaces. METHODS: Streptococcus sanguinis, Streptococcus crista, Streptococcus salivarius, Streptococcus mitis, Actinomyces naeslundii, and Haemophilus parainfluenzae were evaluated as potential antagonists against P. gingivalis ATCC 33277, P. intermedia ATCC 49046, and A. actinomycetemcomitans ATCC 43718 as periodontopathogens. Adhesion of periodontopathogens to the bottom plate of a parallel plate flow chamber was studied in the absence (control) and the presence of pre-adhering antagonistic strains up to a surface coverage of 5%. RESULTS: The largest reduction caused by antagonistic strains was observed for P. gingivalis. All antagonistic strains except S. crista ATCC 49999 inhibited the adhesion of P. gingivalis by at least 1.6 cells per adhering antagonist, with the largest significant reduction observed for A. naeslundii ATCC 51655 (3.8 cells per adhering antagonist). Adhering antagonis...