Two Mannose-resistant Haemagglutinins on Enterotoxigenic Escherichia coli of Serotype O6:K15:H16 or H- Isolated from Travellers' and Infantile Diarrhoea

作者：Cyril J. Smyth · 发表于：Microbiology · 年份：1982 · DOI：10.1099/00221287-128-9-2081 · 被引用次数：146 · 研究领域：Escherichia coli research studies、Immune Cell Function and Interaction、Antimicrobial Peptides and Activities

Enterotoxigenic Escherichia coli of serotype O6:K15:H16 or H have been shown to cause MRHA of bovine erythrocytes (MRHAbov), a characteristic associated with possession of so-called CFA (CFA/II) which facilitates adhesion to the intestinal mucosa. Using putative anti-CFA/II antisera, raised to whole cell vaccines of MRHA+ bov prototype strains of different biotypes with subsequent absorption with their MRHA- bov variants, three immunologically distinct CS antigens were identified on enterotoxigenic strains of this serotype. CS1 antigen was found only on MRHA+ bov isolates of biotype A (rhamnose-negative). Specific anti-CS1 antigen serum agglutinated rhamnose-negative strains, gave a single immunoprecipitate in double immunodiffusion against cell surface extracts of these bacteria, and only inhibited the MRHAbov activity of biotype A strains. The presence of CS1 serologically correlated with the presence of a 16·3 kDal polypeptide band upon SDS-PAGE of extracts. CS2 antigen was identified on MRHA+ bov strains of biotypes B, C and F (rhamnose-positive). Specific anti-CS2 antigen serum agglutinated only the latter strains, gave a single immunoprecipitate against cell surface extracts of only rhamnose fermenting strains, and only inhibited MRHAbov activity of strains of these biotypes. The presence of CS2 antigen serologically was associated with the presence of a 15.3 kDal polypeptide band in SDS-PAGE patterns of extracts. The CS3 antigen was identified by immunodiffusion tests ...